User-Supplied Reagents
|
Consumable |
Supplier |
Lab Designation |
||||||
|---|---|---|---|---|---|---|---|---|
|
Bleach (6–8.5% sodium hypochlorite) |
General lab supplier |
Pre-amp and post-amp |
||||||
|
Deionized water (DI H2O) |
General lab supplier |
Pre-amp and post-amp |
||||||
|
Dimethylsulfoxide (DMSO) |
Sigma, catalog # D8418, or lab grade DMSO CAS# 67-68-5 |
Post-amp, EX only |
||||||
|
EDTA, 0.5 M |
EMD Chemicals, catalog # 4056 Sigma-Aldrich, catalog # E7889 |
Post-amp |
||||||
|
Ethanol, 100% |
General lab supplier |
Post-amp |
||||||
|
Formamide, OmniPur |
VWR, Int'l, catalog # EM-4650 |
Post-amp, non-EX |
||||||
|
Isopropanol (2-propanol), 100% |
General lab supplier |
Post-amp |
||||||
|
Mild detergent, such as Alconox® Powder Detergent |
VWR, Int'l, catalog # 21835 |
Post-amp |
||||||
|
Sodium hydroxide solution to prepare a 0.1 N dilution in DI H2O (for example 10 N, 1 N NaOH) |
General lab supplier |
Pre-amp |
||||||
|
TE, 1X
|
General lab supplier |
Post-amp |
||||||
|
Algecide, such as Aqua-Clear |
VWR, Int'l, catalog # 13197-144 Or Bel-Art Products catalog # F17093-0000 |
Post-amp |
||||||
|
EZ-96 DNA Methylation-Lightning MagPrep |
Zymo Research, catalog # D5046, D5047 |
Pre-amp, for EX Methylation workflows only |
