Recombine DNA

This step creates a combined whole genome and targeted PGx library.

1.

Remove the WGA plate from the Illumina Hybridization Oven after amplification is complete.

2.

Remove the PGx plates from 4°C storage.

3.

Centrifuge the PGx and WGA plates at 280 × g for 1 minute.

4.

Place the PGx plates on bench and carefully remove the seal.

5.

Carefully remove the cap mat from the WGA plate. Set the cap mat aside, upside down and in a safe location, for use later in the protocol.

6.

Transfer 6.5 µl PCR product from the PGx plate directly into the corresponding wells of the WGA plate.

7.

When PGx transfer is complete, mark the WGA checkbox on the barcode label.

8.

Apply Microseal 'B' to the PGx plates and store at -25°C to -15°C for potential future use.