Degas the Te-Flow Heat Exchanger

1. As soon as possible at the beginning of the next day, turn on the water circulator using the power button on the front of the control unit.
2. Remove the water circulator tank cover. Make sure that the tank is filled to within 1 inch from the top of the stainless steel reservoir with DI water.
3. Adjust the temperature to 40°C using the Set button on the control unit screen.

The Set button indicates the selected temperature.

P1 is the internal temperature probe that shows the current temperature of the water bath.

4. Allow the water circulator to reach 40°C before proceeding. This process takes ~15–20 minutes.
1. Close and exit ILASS if open.
2. Power cycle the IAPS with ILASS instrument by turning it off, waiting 30 seconds, and then turning on again.

If the instrument is already off, turn it on.

3. Open the front door and manually move the ROMA arm all the way to the right side of the worktable.
4. After confirming the water circulator has reached 40°C, remove any bubbles trapped in the Te-Flow heat exchanger by manually degassing as follows:
a. Separate the black heat exchanger from the white reagent drip pan underneath by carefully lifting the heat exchanger straight up.

The heat exchanger is very heavy (10.7 Lb).

b. Pivot the heat exchanger upright and away from you into a portrait position with the tubing on the bottom right side.
c. Turn the heat exchanger 90° counter-clockwise into a landscape position. Hold the right side higher to allow bubbles to escape through the tubing in the top-right corner.
d. Rotate the heat exchanger 90° clockwise back to a portrait position.
e. Repeat steps c and d three times for a total of four rotations or until all bubbles are removed (by observing the heat exchanger braided outlet tubing).
f. Using Kimwipes dampened with laboratory-grade water, have an assistant clean all surfaces between the heat exchanger and reagent pan as you hold the heat exchanger. Discard Kimwipes in the appropriate chemical waste container.
g. Carefully and slowly place the heat exchanger back on the reagent drip pan with the tubing on the back right side. Using the two guide pins in the reagent pan, make sure that the heat exchanger is fully seated, even and flush with the reagent pan underneath.
h. Push the entire Te-Flow assembly on Grid 28 towards the back of the IAPS with ILASS until it stops midway on the worktable.
i. Make sure that no kinks are present in the tubing and the tubing is positioned to allow for proper flow.
j. Keep the water circulator turned on to facilitate its rise (via ILASS) to the calibrated set point necessary for the Te-Flow to achieve an operating temperature of 44°C for the X-Stain assay. The water circulator displays the temperature.