Network Connections

Illumina systems are designed to stream data at a regular cadence during the sequencing activity. Depending on the off-load rate, this data transmission could persist for some time after the completion of sequencing. Illumina instruments assume a mostly up network. Network outages could impact data transmission. If a network outage occurs, the instruments are designed to cache all data locally. However, such caching could delay the start of the next sequencing run, depending on storage space on instrument. The instruments are designed to reinitiate data transfer upon restoration of the network.

Review network maintenance activities for potential compatibility risks with the MiSeq i100 Plus System.

For information on the data storage requirements for each file type, refer to Illumina Instrument Control Computer Security and Networking.

Use the following guidelines to install and configure a network connection:

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Use a dedicated connection between the instrument and data management system. Use the Ethernet cable included with the instrument. Make this connection directly or through a network switch.

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A 1 gigabit per second (Gb/s) intranet connection (instrument to network storage and boundary firewall) is required to maintain data transfer times. Lower connection speeds result in reduced instrument availability, increased data transfer times, and might impact sequencing run performance.

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An internet connection is optional.

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Managed switches are recommended.

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Calculate the total capacity of the workload on each network switch. The number of connected instruments and ancillary equipment, such as a printer, can impact capacity.

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If possible, isolate sequencing traffic from other network traffic.

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An unshielded network cable that is 3 m (9.8 ft) long is provided with the instrument for network connections. A CAT-6A cable is recommended for cables longer than 50 m (164 ft).

Use the following recommended network bandwidth per instrument for connections based on 85–90% network efficiency. Primary analysis files include RTA and BCL sequencing output files. Secondary analysis files include on-instrument DRAGEN output files.

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800 megabits per second (Mb/s) (primary only) or ~1 gigabits per second (Gb/s) (primary and secondary) sustained network bandwidth for storing data locally.

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800 Mb/s network bandwidth for uploading primary analysis data to the cloud.

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15 Mb/s network bandwidth for run monitoring or Illumina Proactive Support only.

The MiSeq i100 Plus uses a > 1 Gb/s network connection between the instrument and network storage. Using < 1 Gb/s connection can result in longer copy times or delay the start of subsequent sequencing runs.

Outbound Connections

Connection	Value	Purpose
Port	53	Domain name resolution with customer DNS servers
Port	80	BaseSpace Sequence Hub, or Illumina Proactive configuration
Port	443	Off-instrument control software UI or UCS
Port	8080	BaseSpace Sequence Hub or Illumina Proactive configuration

Inbound Connections

The inbound ports are closed by default. They can be opened in the MiSeq i100 Plus Control Software. Refer to Firewall Settings.

Connection	Value	Purpose
Port	80	Off-instrument control software (certificate)
Port	443	Off-instrument control software (UI)