Consumables Details

The NovaSeq 6000Dx flow cell is a patterned flow cell encased in a cartridge. The flow cell is a glass-based substrate containing billions of nanowells in an ordered arrangement. Clusters are generated in the nanowells from which sequencing is then performed.

Each flow cell has multiple lanes for sequencing pooled libraries. The S2 flow cell has two lanes and the S4 flow cell has four. Each lane is imaged in multiple swaths, and the software then divides the image of each swath into smaller portions called tiles.

Some scratches and other minor cosmetic defects on the flow cell are normal and not expected to compromise data quality and yield. Illumina recommends using these flow cells as per normal.

Flow Cells

1. Flow cell cartridge
2. Four-lane flow cell (S4)
3. Two-lane flow cell (S2)

The underside of each flow cell has multiple gaskets. Libraries and reagents enter the flow cell lanes through the gaskets on the inlet end of the flow cell. Used reagents are expelled from the lanes through the gaskets at the outlet end.

The NovaSeq 6000Dx buffer, cluster, and SBS cartridges have foil-seal reservoirs prefilled with reagents, buffers, and wash solution. Cluster and SBS cartridges are included with NovaSeq 6000Dx reagent kits. The buffer cartridge is sold separately.

The cartridges load directly onto the instrument and are color-coded and labeled to reduce loading errors. Guides in the reagent chiller and buffer drawers ensure proper orientation.

NovaSeq 6000Dx Cartridges

Consumable	Description
Buffer cartridge	Prefilled with sequencing buffers and weighs up to 6.8 kg (15 lb). A plastic handle facilitates carrying, loading, and unloading.   The buffer cartridge contains reagents that are sensitive to light. Keep the buffer container packaged until use.
Cluster cartridge	Prefilled with clustering, indexing, and paired-end reagents and wash solution. Includes a designated position for the library tube. Orange labeling distinguishes the cluster cartridge from the SBS cartridge.   A denaturation reagent in position #30 contains formamide, which is an organic amide and reproductive toxin. To facilitate safe disposal of any unused reagent after the sequencing run, this reservoir is removable.
SBS cartridge	Prefilled with sequencing reagents at volumes specific to the number of cycles the kit supports. Each of the three reagent positions has an adjacent position reserved for the automatic post-run wash. Gray labeling distinguishes the SBS cartridge from the cluster cartridge.   The SBS cartridge contains reagents that are sensitive to light. Keep the SBS container packaged until use.

Three reservoirs are reserved for custom primers and an empty position is reserved for the library tube. For sample traceability, the library tube is loaded into the cluster cartridge during run setup and remains with the cartridge through the end of the run.

Numbered Reservoirs