User-Supplied Consumables and Equipment

Consumables
Consumable	Supplier	Purpose
Centrifuge bottle, 500 ml	General lab supplier	Diluting Tween 20 for a maintenance wash.
Centrifuge tube, 30 ml	General lab supplier	Diluting NaOCl for a maintenance wash.
Disposable gloves, powder-free	General lab supplier	General purpose.
Isopropyl alcohol wipes, 70% or Ethanol alcohol wipes, 70%	VWR, catalog # 95041-714, or equivalent General lab supplier	Cleaning components before a run and general purpose.
Lab tissue, low-lint	VWR, catalog # 21905-026, or equivalent	Drying the flow cell stage and general purpose.
Reagent grade NaOCl, 5%	Sigma-Aldrich, catalog # 239305	Performing a maintenance wash.
Pipette tips, 2 μl	General lab supplier	Pipetting for diluting and loading libraries.
Pipette tips, 20 μl	General lab supplier	Pipetting for diluting and loading libraries.
Pipette tips, 200 μl	General lab supplier	Pipetting for diluting and loading libraries.
Pipette tips, 1000 μl	General lab supplier	Pipetting for diluting and loading libraries.
Reagent or spectrophotometric-grade isopropyl alcohol (99%), 100 ml bottle	General lab supplier	Cleaning optics components periodically and support the objective cleaning cartridge.
Tween 20	Sigma-Aldrich, catalog # P7949	Performing a maintenance wash.
Water, laboratory-grade	General lab supplier	Diluting Tween 20 and sodium hypochlorite for a maintenance wash.

Equipment
Item	Source
Freezer, -25°C to -15°C	General lab supplier
Graduated cylinder, 500 ml, sterile	General lab supplier
Ice bucket	General lab supplier
Pipette, 20 µl	General lab supplier
Pipette, 200 µl	General lab supplier
Pipette, 1000 µl	General lab supplier
Refrigerator, 2°C to 8°C	General lab supplier
Tub, water baths*	General lab supplier

* Use a tub that can accommodate two reagent cartridges and the appropriate water level. For example, (61 cm × 91.4 cm × 25.4 cm)(24 in × 36 in × 10 in).