NextSeq 1000/2000 Integration v2.1 Validation and Troubleshooting

This section explains how to validate the installation of the Illumina NextSeq 1000/2000 Integration Package v2.1.

The validation process involves:

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Running samples through the Library Prep Validation v2.0.3 workflow.

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The workflow contains a single-step protocol that models the library prep workflow required to produce libraries tagged with index sequences. At the end of the step, these libraries are routed to NextSeq 1000/2000 Sequencing v2.0 workflow.

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Running the libraries through the NextSeq 1000/2000 Sequencing v2.0 workflow. This process validates the following information:

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Successful sequential step advancement of samples in the following steps:

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Automatic validation of run setup information before sample sheet generation or planned run creation.

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Automated generation of a sample sheet. The sample sheet is used to start the sequencing run on NextSeq 1000/2000 Control Software via Local run mode.

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Automated creation of a planned run on Illumina Connected Analytics (ICA). The planned run created is retrieved by NextSeq 1000/2000 Control Software to start run via Hybrid or Cloud run mode.

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Automated tracking of the NextSeq 1000/2000 sequencing run and parsing of run statistics from BaseSpace Sequence Hub (BSSH) into Clarity LIMS using SIS Integration Service.

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Automated tracking of the secondary analysis using DRAGEN (up to BCL Convert only) and parsing of demultiplexing metrics from BaseSpace Sequence Hub (BSSH) into Clarity LIMS using SIS integration service.

Before running the validation steps below, these steps assume that the NextSeq 1000/2000 Sequencing v2.0 Integration Package is installed and you have imported the default Clarity LIMS configuration.

For information on how the integration works, refer to NextSeq 1000/2000 Integration v2.1 Configuration and User Interaction.