User-Supplied Equipment

Equipment

Supplier

Lab Designation

Adapters to centrifuge 96-well plates and tubes (2 sets)

General lab supplier

Pre-amp and post-amp

Autodesiccator cabinet

VWR, Int'l, catalog # 74950-342

Post-amp

Cap mat sealer (recommended)

Corning, catalog # 3081

Pre-amp and post-amp

Centrifuge, benchtop 110 V (US), 220 V (EU)

General lab supplier

Pre-amp

Centrifuge, benchtop refrigerated 8–3000 × g 110 V (US), 220 V (EU)

General lab supplier

Post-amp

Forceps

• Included with system, only needed if additional pairs are required

VWR, Int'l, catalog # 25601-008

Post-amp

Magnetic Stand

ZR-96 MagStand, catalog # P1005

Pre-amp, for Methylation workflows only

Micropipettors

• 2 separate sets (1 for pre- and 1 for post-amplification processes):
• P-20
• P-200
• P-1000

General lab supplier

Pre-amp and post-amp

Multichannel precision pipettes

• 1 set: P-1000
• 2 separate sets (1 for pre- and 1 for post-amplification processes):
• P-20
• P-200
• P-1000

General lab supplier

Pre-amp and post-amp

Nalgene™ rectangular autoclavable PPCO carboy with spigot (10 L)

Thermo Fisher Scientific, 2319‑0020

Post-amp

NIST Thermometer

VWR, Int'l , catalog # 61161-364

Post-amp, EX-only

Optical tachometer/stroboscope (recommended)

Cole-Parmer, catalog # A-87700-06

Post-amp

Plates, 96-well, black, flat bottom FLUOTRAC 200

Greiner, catalog #655076

Pre-amp,

Optional for PicoGreen Quantification

Reservoir, 96-Bottom Trough, 300 ml
• Axygen catalog # RES-SW96-HP-SI

Pre-amp, automated protocol only

Infinium Amplification System

Reservoirs

• Full, 150 ml
• Half, 75 ml
• Quarter, 40 ml
• Beckman Coulter, catalog # 372784
• Beckman Coulter, catalog # 372786
• Beckman Coulter, catalog # 372790

Post-amp, automated protocol only

Infinium Automated Pipetting System

Reservoir Frame

Beckman Coulter, catalog #372795

Post-amp, automated protocol only

Infinium Automated Pipetting System

Serological pipette aid, 2

General lab supplier

Pre-amp and post-amp

Stop watches/timers

• 2 separate sets: 1 for pre- and 1 for post-amplification processes

General lab supplier

Pre-amp and post-amp

Thermal cycler with the following specification

• Heated lid
• Max Ramp Rate:  5°C / sec
• Average Ramp Rate:  3.3°C / sec
• Temp Range:  0-100°C
• Temperature Accuracy:  +/- 0.2°C (at 90°C)
• Temperature Uniformity:  +/- 0.4°C well-to-well

General lab supplier

Post-amp, Infinium Pharmacogenomics protocol only

Pre-amp, for Methylation workflows

 

Post-amp, for Infinium Pharmacogenomics protocol

Thermocycler for bisulfite conversion

• Temp Range:  0-100°C
• Sample volume: 150 ul

General lab supplier

Pre-amp, for Methylation workflows only

Tube rack

• 2 separate sets: 1 for pre- and 1 for post-amplification processes
• Any rack fitting the Infinium reagent 17 mm tube diameter

VWR, Int'l, catalog # 66023-540

Pre-amp and post-amp

Tube vortexers

• 2 separate sets: 1 for pre- and 1 for post-amplification processes

General lab supplier

Pre-amp and post-amp

Vacuum source, hose, or pump capable of pulling greater than 508 mm Hg

General lab supplier

Post-amp

Vacuum desiccator tubing (included in Infinium Starter Kit, only needed if additional repairs are required)

VWR, Int'l , catalog # 62995-335

Post-amp

Vacuum desiccator racks (included in Infinium Starter Kit, only needed if additional repairs are required)

VWR, Int'l , catalog # 66023-526

Post-amp

[Optional] Vacuum gauge for vacuum desiccator

General lab supplier

Post-amp

[Optional] High-capacity desiccator

General lab supplier

Post-amp, automated protocol only