User-Supplied Materials

Material

Supplier

Lab Designation

Absorbent pads/towels

General lab supplier

Pre-amp and post-amp

Adhesive seals (Microseal 'B')

Bio-Rad, catalog # MSB-1001

Pre-amp (PGx only)

Aluminum foil

General lab supplier

Pre-amp

Cap mats, 96-well, pierceable, nonautoclavable

Thermo Fisher Scientific, catalog # AB-0566

Pre-amp and post-amp

Compressed air can

VWR, Int'l, catalog # 16650-027

Post-amp

Containers

• 100 ml
• 200 ml
• 1 L, for diluting bleach
• 2 containers capable of holding polypropylene test tube racks

General lab supplier

Post-amp

Foil adhesive seals

Beckman Coulter, catalog # 538619

For post-amp EX-only

Glass bottle for DMSO preparation

General lab supplier

For post-amp EX-only

Infinium Amplification System (IAS) tips

• 50 µl Non-Filtered Conductive Tips
• 300 µl Non-Filtered Conductive Tips
• 1000 µl Non-Filtered Conductive Tips

 

Hamilton catalog # 235966

Hamilton catalog # 235902

Hamilton catalog # 235904

For pre-amp EX-only

IAS waste bags (L × W × H)

• Left (96 CO-RE MPH tips) 27 in × 8 in × 22 in
• Right (1000 µl channel tips) 14.5 in × 8.2 in × 18 in

General lab supplier

Pre-amp

Heat sealing foil sheets, Thermo-Seal

Thermo Fisher Scientific, catalog # AB-0559

Post-amp

Kimwipes or any lint-free tissue

General lab supplier

Pre-amp and post-amp

Lab coats

• 2 supplies: 1 for pre- and 1 for post-amplification processes

General lab supplier

Pre-amp and post-amp

Pipette tips

• 10 µl aerosol filter
• 20 µl aerosol filter
• 200 µl aerosol filter
• 1000 µl aerosol filter

General lab supplier

Pre-amp and post-amp

Pipettes, serological, 10–50 ml

General lab supplier

Pre-amp and post-amp

Pipetting troughs, disposable

VWR, Int'l, catalog # 21007-970

Pre-amp and post-amp

Polypropylene test tube racks (recommended)

Cole-Parmer, catalog # EW-06739-17

Post-amp

Powder-free gloves

• 2 supplies: 1 for pre- and 1 for post-amplification processes

General lab supplier

Pre-amp and post-amp

ProStat EtOH presaturated wipes

• Recommend 1 wipe per 2 chips; 30 wipes per package
• Substitute with Kimwipes and 70% EtOH

Contec, catalog # PS-911EB/EtOH

Pre-amp and post-amp

Safety glasses

• 2 supplies: 1 for pre- and 1 for post-amplification processes

General lab supplier

Pre-amp and post-amp

Skirted microplates, 96-well, 0.2 ml

• Microseal 96-well skirted polypropylene microplates, 8x12 well array
• TCY plates, 1 per run
• 0.8 ml storage plate (MIDI plate), conical well bottom

Bio-Rad, catalog # MSP-9601

 

Thermo Fisher Scientific, catalog # AB0765

Pre-amp. Any may be used for manual workflows. EX automation uses only MIDI plate.

Skirted PCR microplates, 96-well, 200 µl

Bio-Rad, catalog # HSP-9601

PGx amplification plate, Infinium Pharmacogenomics protocol only

Storage microplates, 96-well, 0.8 ml

MIDI plates, 1 per run

Thermo Fisher Scientific, catalog # AB0765

DNA storage in pre-amp

Tubes

• 15 ml conical
• 50 ml conical

General lab supplier

Pre-amp and post-amp