Cleaning the Infinium Automated Pipetting System with Illumina Automation Control

Perform this activity once a week.

1.

At the beginning of each day, check for any leaks.

2.

In the Illumina Automation Control Software, select Sys Init.

3.

Select Tips Up.

4.

Fold a Kimwipe in half lengthwise and soak it with 70% EtOH.

5.

Fold the Kimwipe around the IAPS with IAC tip.

6.

Starting from one end of the tip row, wipe each tip gently along the entire lower half of the tip.

7.

Reverse the Kimwipe to use the other, fresh side.

8.

Starting from the opposite end of the tip row, wipe each tip a second time along the entire lower half.

9.

Clean the waste station with the Kimwipe.

Perform this activity annually or whenever mold or bacterial growth is detected in the robot fluidics.

1.

In the carboy, prepare a 1 L 10% bleach solution by adding 100 ml bleach to 900 ml DI H2O.

2.

Select Robot QC Tasks | Robot Bleach Wash.

3.

In the Basic Run Parameters pane, enter 1 for a system wash.

4.

Select Run.

5.

When prompted, swap out the system liquid carboy for the carboy containing 10% bleach solution.

6.

Select OK.

7.

When prompted, swap out the bleach solution with the system liquid carboy.

8.

Select OK.

9.

Observe lines for air bubbles.

10.

Run additional washes (SysWash) until the lines are free of air bubbles. If bubbles persist in the lines, contact Illumina Technical Support.