Fragment DNA

This step enzymatically fragments the DNA. An endpoint fragmentation is used to prevent overfragmentation.

• FMS
• Cap mats
1. Preheat the heat block with the MIDI plate insert to 37°C.
2. Prepare the following consumable:

Item

Storage

Instructions

FMS

‑25°C to ‑15°C

Thaw at room temperature and invert to mix. Pulse centrifuge at 280 × g for 1 minute.

RA1

‑25°C to ‑15°C

If resuspending the MSA4 plate today, remove the RA1 from the freezer and thaw at room temperature.
1. Pulse centrifuge the MSA4 plate at 280 × g.
2. Remove the cap mat.
3. At the robot PC, select MSA4 Tasks | Fragment MSA4.
4. Place the MSA4 plate on the robot bed according to the bed map. Remove the cap mat.

Robot Setup for Fragment MSA4

  1. FMS
  2. MSA4 Plate
5. Place FMS tubes in the robot tube rack according to the bed map. Remove the cap mat.
6. At the robot PC, select Run.
7. When the process completes, select OK.
8. Remove the plate from the robot bed and seal with a cap mat.
9. Vortex at 1600 rpm for 1 minute.
10. Pulse centrifuge at 280 × g.
11. Incubate the sealed plate on the 37°C heat block for 1 hour.

If you are continuing, leave the plate in the 37°C heat block until you have completed preparation for the next step. Do not leave the plate on the heat block for longer than 2 hours.

SAFE STOPPING POINT

If you are stopping, seal the plate(s), and store at ‑25°C to ‑15°C.