Resuspend DNA

This step uses RA1 to resuspend the precipitated DNA.

This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation, ingestion, skin contact, and eye contact. Wear protective equipment, including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in accordance with applicable regional, national, and local laws and regulations. For additional environmental, health, and safety information, refer to the SDS at support.illumina.com/sds.html.

• RA1
• Foil heat seals
1. If the MSA4 plate was stored at -25°C to -15°C, thaw at room temperature, and then remove and discard the cap mat.
2. Prepare the following consumable:

Item

Storage

Instructions

RA1

-25°C to ‑15°C

Thaw at room temperature and invert to mix.
3. Preheat the Illumina Hybridization Oven to 48°C.
4. Preheat the heat sealer for at least 20 minutes before use.
1. At the robot PC, select MSA4 Tasks | Resuspend MSA4.
2. Place the MSA4 plate on the robot bed according to the bed map.

Robot Setup for Resuspend DNA

A. RA1 in Quarter Reservoir
B. MSA4 Plate
3. Place a quarter reservoir in the reservoir frame according to the robot bed map, and add RA1 as follows:
• For 48 samples, add 4.5 ml RA1
• For 96 samples, add 9 ml RA1
4. At the robot PC, select Run.
5. When prompted, select OK.
6. Remove the MSA4 plate from the robot worktable.
7. Apply a foil seal to the MSA4 plate using the heat sealer.
8. Incubate in the preheated Illumina Hybridization Oven for 1 hour.
9. Vortex the plate at 1800 rpm for 1 minute.
10. Check to make sure that the pellets are resuspended. If necessary, repeat the incubation and vortexing steps.
11. Pulse centrifuge at 280 × g.

SAFE STOPPING POINT

If you are stopping, store sealed MSA4 plate(s) at 2°C to 8°C for up to 24 hours. If more than 24 hours, store at -25°C to -15°C.

Store sealed RA1 at ‑25°C to ‑15°C. If using RA1 the next day, seal it, and store it overnight at 4°C.