Nonpolymorphic Controls

Nonpolymorphic controls test the overall performance of the assay, from amplification to detection, by querying a particular base in a nonpolymorphic region of the bisulfite genome. These controls compare assay performance across different samples. One nonpolymorphic control has been designed to query each of the four nucleotides (A,T, C, and G). The target with the C base results from querying the opposite whole genome amplified strand generated from the converted strand.