Automated Protocol

This section describes pre- and post-amplification automated laboratory protocols for the Infinium HD Methylation Assay using IAPS with Illumina Automation Control (IAC). The protocol includes additional information and optional steps for using the Illumina LIMS to track barcodes and other project information. Follow the protocols in the order shown. For information on using LIMS, refer to the Illumina LIMS Software Guide (document # 1000000032951).

Before proceeding, confirm the kit contents and make sure that you have the necessary consumables and equipment, including bisulfite conversion reagents and HD FFPE DNA Restore kits (if desired).
Follow the protocol in the order described using the specified parameters. Unless a safe stopping point is specified, move immediately to the next step.