Manual Protocol

This section describes pre- and post-amplification manual laboratory protocols for the Infinium HD Methylation Assay.

Before proceeding, confirm the kit contents and make sure that you have the necessary consumables and equipment, including bisulfite conversion reagents and HD FFPE DNA Restore kits (if desired).
Follow the protocol in the order described using the specified parameters. Unless a safe stopping point is specified, move immediately to the next step.