Revision History

Added semi-automated protocol.

Added acronyms.

Updated storage temperature for RA1.

Removed redundant steps.

Clarified steps for preparing the robot and setting up the chamber rack.

Updated the manual and automated workflow figures to reflect the proper incubation time.

Updated kit references in Convert DNA.

Updated the Methylation kit to the Zymo EZ-96 DNA Methylation Lightning MagPrep Kit. The HD and HTS methylation protocols now use the same kit.

Updated the protocol to reflect the changes for using the Lightning MagPrep Kit.

Replaced the figures in the Wash BeadChip section with figures showing the 8-sample BeadChips.

Updated the Convert DNA sections to reflect the updated manual and automatic workflows.

Added content to Hybridize DNA to the BeadChip sections for use of 12x1 BeadChip.

Updated images in Hybridize DNA to the BeadChip and Wash BeadChips sections to reflect appropriate BeadChip layouts.

Updated introduction to reflect use of the assay with NextSeq 550Dx, IAPS, additional information on Infinium probes, and other minor updates.

Updated styles to new documentation format.

Removed incorrect repetition from Single-Base Extension flow-through chamber fill steps for RA1, XC1, XC2, XC3, and formamide.

Added step to wait five minutes to Stain BeadChips following third XC3 step.

Removed all references to WG# in barcode information in regards to BCD plates and MSA plates.

Added Barcode Numbers section to Tips and Techniques to explain the new barcode numbering scheme.

Corrected Reagent Reuse in Tips and Techniques. XC4 is reusable not LX2.

Corrected storage temperatures of MA1, PB1, PM1, XC3, and XC4.

Corrected reagents listed in first and last bullets of About Reagents section in the Extend and Stain introduction.

Changed document name to Infinium HD Methylation Assay Reference Guide.

Document updated to reflect latest style and formatting standards.

Added Preparation and Storage of User-Supplied Reagent section to consolidate reagent preparation and use instructions.

Reference to Experienced User Cards replaced with Infinium HD Methylation Assay Checklist Manual Protocol (document # 1000000077298) and Infinium HD Methylation Assay Checklist Automated Protocol (document # 1000000077299) which provide current format of experienced user instructions.

Added list of acronyms.

Consumables and equipment information moved to Infinium Assay Lab Setup and Procedures Guide (document # 11322460).

Added Tip and Techniques section, includes:

Updated the consumables table in Hybridize to BeadChip section to reflect the addition of a third tube of PB2 that is included in the Methylyation EPIC Kit (96 Samples) to provide enough volume to process 96 samples.

Indicated that up to 24 BeadChips can be washed with 550 ml of PB1.

Incorporation of LIMS support.