Introduction to Infinium LCG Assay
The Illumina Infinium LCG Assay transforms DNA analysis by streamlining sample preparation and enabling high multiplexing. Using Infinium I and II probe designs and a dual-color channel approach, the Infinium LCG Assay enables DNA analysis of up to several million SNPs and CNV markers per sample.
The Infinium LCG Assay accomplishes this unlimited
multiplexing by combining whole-genome amplification (WGA) sample
preparation with direct, array-based capture, and enzymatic scoring of the
SNP loci. Locus discrimination or CNV determination comes from a combination of high bead type representation per feature, sequence-specific hybridization capture, and
array-based, single-base primer extension. With the Infinium I probe design, the 3' end of the primer overlaps with
the SNP site. If there is a perfect match, extension occurs and signal is
generated. If there is a mismatch, extension does not occur, and no signal is
generated. With the Infinium II probe
design, the 3' end of the primer is positioned directly adjacent to the SNP
site, or if a nonpolymorphic probe, directly adjacent to the nonpolymorphic site.
Allele-specific, single-base extension of the primer incorporates a biotin nucleotide or a dinitrophenyl labeled nucleotide. C and G nucleotides are biotin labeled. A and T nucleotides are dinitrophenyl labeled. Signal amplification of the incorporated label further improves the overall signal-to-noise ratio of the assay.
The Infinium LCG Assay offers:
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High call rate and accuracy |
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Unlimited genomewide marker selection |
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Single tube amplification, single chip, no PCR |
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Minimal risk of carryover contamination |
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Low DNA input—200 ng per sample |
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Walk-away automation using the Infinium Automated Pipetting System with Illumina Automation Control. |
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Compatibility with Illumina iScan systems |
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Multiple sample BeadChip format |
