Resuspend DNA

This step uses RA1 to resuspend the precipitated DNA.

• RA1
• Foil heat seals
• The RA1 volume used for DNA resuspension allows for samples to be hybridized at least in duplicate if needed. Samples are resuspended with 46 µl RA1 total, using 26µl per sample inlet of the BeadChip.
• Dispense only the volume necessary for each step. The Extend and Stain BeadChips procedure also requires RA1.
• Use fresh RA1 for each step that requires it. Properly stored RA1 that has not been dispensed for this resuspension step or the extend and stain step is considered fresh.
• RA1 might form visible precipitate or crystals. Before each use, hold in front of a light and inspect. Invert several times to redissolve the solution as needed.

This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation, ingestion, skin contact, and eye contact. Wear protective equipment, including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in accordance with applicable regional, national, and local laws and regulations. For additional environmental, health, and safety information, see the SDS at support.illumina.com/sds.html.

1. If the MSA6 plate was stored at -25°C to -15°C, thaw at room temperature, and then remove and discard the cap mat.
2. Prepare the following consumable:

Item

Storage

Instructions

RA1

-25°C to ‑15°C

Thaw at room temperature and invert to mix.
3. Preheat the Illumina Hybridization Oven to 48°C.
4. Preheat the heat sealer for at least 20 minutes before use.
1. Add 46 µl RA1 to each well of the MSA6 plate.
2. With the dull side facing down, apply a foil heat seal to the plate. Hold the heat sealer sealing block down firmly and evenly for 5 seconds.
3. Incubate at 48°C in the preheated Illumina Hybridization Oven for 1 hour.
4. Vortex at 1800 rpm for 1 minute, and then pulse centrifuge at 280 x g for 1 minute.
5. Repeat steps 4 and as needed to resuspend the pellets.
6. [Optional] Unless you are stopping, you can set aside the MSA6 plate for up to 1 hour.

SAFE STOPPING POINT

If you are stopping, seal the plate and store at ‑25°C to ‑15°C for up to 24 hours.

Store sealed RA1 at ‑25°C to ‑15°C.