Illumina LIMS

If you are using the automated protocol with Illumina LIMS, use the following guidelines:

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At each step in the protocol, make sure that the Use Barcodes checkbox is selected.

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The barcode must be on the right side of the plate. Make sure the barcode label fits between the notches and does not cover the holes on the top of the plate.

Correctly Placed Barcodes

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When prompted, enter the number of samples, plates, or BeadChips, and then select OK.

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Each time you select Run to start a new process, you are prompted to log in to LIMS.

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If prompted to select the project and the batch ID or DNA plate, perform one of the following options:

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Select your current project. The available batches appear in the Sample Batch ID pane. Select a batch to see the associated DNA plate appear in the DNA Plates pane.

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Use Search to search for a specific Batch ID or DNA plate.

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Some steps require verification in LIMS before you can start.

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If verification is successful, a blue confirmation message appears at the top of the window.

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If the verification fails, a red error message appears at the top of the window. Do not proceed. Instead, use the following steps to troubleshoot the problem:

Select the Reports tab in the upper-right corner.

In the left pane, select Tracking Reports | Get Queue Status.

Scan the plate barcode, and select Go.

Note which step the plate is queued to run, and proceed with that step.