Edit the Sample Sheet for Custom Primers
You can set up your sample sheet for custom primers using Illumina Experiment Manager v1.2, or later. If you edit your sample sheet manually, list the custom primers in the Settings section of the sample sheet.
1. | Open your sample sheet for editing in Excel or Notepad. |
2. | Locate the Settings section. If your sample sheet does not have a Settings section, create one after the Reads section and before the Data section. |
3. | In the Settings section, add a line for each custom primer using the following example. |
Settings |
|
---|---|
CustomRead1PrimerMix |
C1 |
CustomIndexPrimerMix |
C2 |
CustomRead2PrimerMix |
C3 |
Include C1 for the Read 1 primer, C2 for the Index Read primer, and C3 for the Read 2 primer.
If you are using a combination of Illumina primers and custom primers, list only the custom primer you plan to use.