Denature and Dilute Libraries

If necessary for your library type, denature and dilute libraries, and add optional PhiX control. Refer to MiSeq System Denature and Dilute Libraries Guide (document # 15039740). If you are performing the VeriSeq PGS workflow, refer to the VeriSeq PGS Library Preparation Guide (document # 15052877).

This step does not apply to all library types. Some Illumina sample preparation methods result in a ready-to-use normalized concentration of pooled libraries. Refer to the sample preparation guide for the kit used to prepare sample libraries.