Flow Cell

The MiSeq is a one-time use substrate constructed from glass, serving as the platform for cluster generation and the sequencing process.

Reagents enter the flow cell through the inlet port, pass through the single-lane imaging area, and then exit the flow cell through the outlet port. Waste exiting the flow cell is delivered to the waste bottle.

Libraries are loaded onto the reagent cartridge before setting up the run, and then automatically transferred to the flow cell after the run begins.

Flow Cell Cap Color

The cap color of the flow cell container indicates the flow cell type:

Flow Cell	Flow Cell Cap Color
Standard Flow Cell PGS Flow Cell	Clear/White
Micro Flow Cell	Green
Nano Flow Cell	Yellow