Load the Reagent Cartridge

1.

Open the reagent chiller door.

2.

Hold the reagent cartridge on the end with the Illumina label and slide the reagent cartridge into the reagent chiller until the cartridge stops.

Always use the reagent cartridge associated with the type of flow cell that you loaded. If the reagent cartridge is not compatible, a message appears on the screen. Select Back to load the appropriate reagent cartridge or Home to return to the Home screen.

3.

Close the reagent chiller door.

4.

If the software does not identify the RFID of the reagent cartridge, refer to Troubleshooting RFID Not Recognized Error on the MiSeq.

5.

If the reagent cartridge is not compatible with the flow cell, a message appears. Select Back to load a compatible cartridge, or select Exit to return to the Home screen.

In the event the RFID cannot be read, perform the following steps:

a.

Open and then close the reagent chiller door to retry the RFID read.

b.

If the RFID fails a second time, select Enter Reagent Kit Barcode.

c.

Enter the reagent kit barcode number, which is on the kit label, directly below the barcode.

d.

Enter the reagent kit part number, which is located to the right of REF on the kit label.

e.

Select Next to return to the Load Reagents screen.

f.

Select Next to proceed to the next run setup step.

6.

Close the reagent compartment door.

7.

Select Next.