Load the Flow Cell

Make sure that you have washed and thoroughly dried the flow cell.

1. Raise the flow cell compartment door, and then press the release button to the right of the flow cell clamp.

The flow cell clamp opens.

Open Flow Cell Clamp

2. Make sure that the flow cell stage is free of lint. If lint or other debris is present, clean the flow cell stage using an alcohol wipe or a lint-free tissue moistened with ethanol or isopropanol. Carefully wipe the surface of the flow cell stage until it is clean and dry.
3. Holding the flow cell by the edges, place it on the flow cell stage.

Place Flow Cell on Stage

4. Gently press down on the flow cell clamp to close it over the flow cell.

As the flow cell clamp closes, alignment pins position the flow cell. An audible click indicates that the flow cell clamp is secure.

Close Flow Cell Clamp

5. If the software does not identify the flow cell RFID, refer to Resolve RFID Read Failure.

If the RFID cannot be read, perform the following steps:

a. Open and then close the flow cell compartment door to retry the RFID read.
b. If the RFID fails a second time, select the keyboard icon to open the on-screen keyboard.
c. Enter the barcode number of the flow cell, which is located on the flow cell container label directly below the barcode. Select Done.
d. Select Next to proceed to the next run setup step.
6. Close the flow cell compartment door.
7. Select Next.