Run Metrics

Run metrics appear on the Sequencing screen at different points in a run. During cluster generation steps, no metrics appear.

After sequencing begins, the following metrics appear at the indicated cycles:

Metric

Kit

Cycle

Intensity

MiSeq Reagent Kits, v3

MiSeq Reagent Kits, v2

Cycle 1–7

Cycle 1–4

Cycle 1–4

Intensity and Cluster Density

MiSeq Reagent Kits, v3

MiSeq Reagent Kits, v2

Cycle 8–25

Cycle 5–25

Cycle 5–25

Intensity, Cluster Density, % PF, Yield, and Q-scores

MiSeq Reagent Kits, v3

MiSeq Reagent Kits, v2

Cycle 26 through run completion

For MiSeq run specifications, visit the MiSeq System specifications page on the Illumina website.