Review Pre-Run Check

The system performs a check of all run components, disk space, and network connections before starting the run.

If any items do not pass the pre-run check, a message appears on the screen with instructions on how to correct the error. For more information, refer to Resolve Run Setup Errors.

When all items successfully pass the pre-run check, select Start Run.

Important Notes Before Starting the Run

The MiSeq is sensitive to vibration. Touching the instrument after starting a run could adversely affect sequencing results.

After selecting Start Run, do not open the flow cell compartment or the reagent compartment doors, or touch the instrument monitor except to pause the run. For more information, refer to Pause a Run.

Make sure to close all files on the MiSeq before starting a run. Do not open files during a run.