Pause or Stop a Run

You can temporarily pause a run before it has completed. This action is useful when addressing potential issues, such as a full waste bottle. Paused runs can be resumed.

When you select Pause, the current command is completed before pausing the run and placing the flow cell in a safe state.

To pause a run from the Sequencing screen, select Pause. The button changes to Resume. When you are ready to resume the run, select Resume.

You can stop a run during sequencing before the run before its completion by using the Stop button located on the sequencing screen. You might stop a run if the run was set up incorrectly, if the data quality is bad, or hardware malfunctions.

When a run is stopped, the current command is not completed, and the flow cell stage moves to the forward position. Real-Time Analysis software continues analysis for the most recently completed cycle.

Stopping a run is final. A stopped run cannot be resumed. The only option is to proceed to an instrument wash.