User-Supplied Consumables and Equipment
Make sure that the following user-supplied consumables are available before beginning a run.
|
Consumable |
Supplier |
Purpose |
|---|---|---|
|
Stock 1.0 N NaOH, molecular biology grade |
General Lab supplier |
Denaturing sample libraries and PhiX control DNA |
|
Alcohol wipes, 70% Isopropyl or Ethanol, 70% |
VWR, catalog # 95041-714*
General lab supplier |
Cleaning the flow cell holder |
|
Disposable gloves, powder-free |
General lab supplier |
General use |
|
Lab tissue, low-lint |
VWR, catalog # 21905-026* |
Cleaning the flow cell stage and the foil seal covering the load samples reservoir |
|
Lens paper, 4 x 6 in |
VWR, catalog # 52846-001* |
Cleaning the flow cell |
|
Microcentrifuge tubes |
General lab supplier |
Denaturing and diluting sample libraries and PhiX control DNA |
|
MiSeq tubes |
Illumina, |
Washing the template line, for use with the VeriSeq PGS workflow (optional for other workflows) |
|
NaOCl, 5% |
Sigma-Aldrich, catalog # 239305* |
Washing the template line, for use with the VeriSeq PGS workflow (optional for other workflows) |
|
10 mM Tris-HCl, pH 8.5 |
General lab supplier |
Diluting libraries and an optional PhiX control before denaturation. |
|
200 mM Tris-HCl, pH 7.0 |
General lab supplier |
Neutralizing libraries and an optional PhiX control after denaturation |
|
Tween 20 |
Sigma-Aldrich, catalog # P7949 |
Washing the instrument |
|
Tweezers, square-tip plastic (optional) |
McMaster-Carr, catalog # 7003A22* |
Removing flow cell from flow cell shipping container |
|
Water, laboratory-grade |
General lab supplier |
Washing the instrument |
* or laboratory-grade equivalent
