User-Supplied Consumables & Equipment
The following section provides information on the required user-supplied consumables and equipment.
The MiSeq i100 Series system features a touch screen monitor for configuration and run management, but you can also connect a USB keyboard and mouse via the USB 2.0 ports. Refer to Peripheral Connections.
Consumables
|
Consumable |
Supplier |
Purpose |
|---|---|---|
|
Air filter |
Illumina, catalog # 20116201 |
Replacing the air filter. MiSeq i100 ships with two air filters, one preinstalled and one spare. |
|
Re-useable test dry cartridge |
Illumina, catalog # 20102505 |
Performing a system check. MiSeq i100 ships with one re-useable test dry cartridge. |
|
Re-useable test wet cartridge |
Illumina, catalog # 20102509 |
Performing a system check. MiSeq i100 ships with one re-useable test wet cartridge. |
|
Disposable gloves, powder-free |
General lab supplier |
General purpose. |
|
Drip tray pad |
Illumina, catalog # 20116211 |
Replacing the drip tray pad. |
|
Waste bottle |
Illumina, catalog # 20116206 |
Replacing the waste bottle. MiSeq i100 ships with one waste bottle. |
|
Microcentrifuge tube, 1.5 ml |
VWR, catalog # 20170-038, or equivalent |
Combining volumes when preparing the library. |
|
Pipette tips, 20 μl |
General lab supplier |
Pipetting for diluting and loading libraries. |
|
Pipette tips, 200 μl |
General lab supplier |
Pipetting for diluting and loading libraries. |
|
Pipette tips, 1000 μl |
General lab supplier |
Pipetting for diluting and loading libraries. |
|
[Optional] PhiX Control v3 |
Illumina, catalog # FC-110-3001 |
Spiking in PhiX control for kits with 600 cycles or less. |
|
[Optional] PhiX Indexed Control (1000 cycles) |
Illumina, catalog # 20151542 |
Spiking in PhiX control for kits with 1,000 cycles. |
|
[Optional] HT1 (Hybridization Buffer) |
Illumina, catalog #20015892 |
Reagent used to dilute denatured libraries prior to sequencing. |
Equipment
|
Item |
Source |
|---|---|
|
Microtube centrifuge |
General lab supplier |
|
Pipette, 20 µl |
General lab supplier |
|
Pipette, 200 µl |
General lab supplier |
|
Pipette, 1000 µl |
General lab supplier |
|
Vortex mixer |
General lab supplier |
|
[Optional] USB keyboard |
General supplier |
|
[Optional] USB mouse |
General supplier |
