Ancillary Equipment
Purchase the following equipment for sequencing purposes.
Item |
Source |
Purpose |
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General lab supplier |
Storing the cartridge. |
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Centrifuge |
General lab supplier |
Diluting libraries to the loading concentration. |
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Ice bucket |
General lab supplier |
Setting aside libraries until sequencing. |
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Pipette, 10 µl |
General lab supplier |
Diluting libraries to the loading concentration. |
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Pipette, 20 µl |
General lab supplier |
Diluting libraries to the loading concentration and loading libraries into the cartridge. |
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Pipette, 200 µl |
General lab supplier |
Diluting libraries to the loading concentration. |
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Refrigerator, 2°C to 8°C |
General lab supplier |
Storing the flow cell or thawing the cartridge. |
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Vortexer |
General lab supplier |
Diluting libraries to the loading concentration. |
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[Optional] One of the following temperature-controlled water baths or equivalent that can hold at 25°C:
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Thawing the cartridge. |
The following equipment is used to denature libraries that have been normalized.
Equipment |
Supplier |
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Centrifuge |
General lab supplier |
Vortexer |
General lab supplier |
For information on how to manually denature and dilute prepared libraries for sequencing on NextSeq 1000/2000, refer to either Standard SBS Sequencing Protocol or XLEAP-SBS Sequencing Protocol.