Ancillary Equipment

Equipment for Sequencing

Purchase the following equipment for sequencing purposes.

Item

Source

Purpose

Freezer, -25°C to -15°C

General lab supplier

Storing the cartridge.

Centrifuge

General lab supplier

Diluting libraries to the loading concentration.

Ice bucket

General lab supplier

Setting aside libraries until sequencing.

Pipette, 10 µl

General lab supplier

Diluting libraries to the loading concentration.

Pipette, 20 µl

General lab supplier

Diluting libraries to the loading concentration and loading libraries into the cartridge.

Pipette, 200 µl

General lab supplier

Diluting libraries to the loading concentration.

Refrigerator, 2°C to 8°C

General lab supplier

Storing the flow cell or thawing the cartridge.

Vortexer

General lab supplier

Diluting libraries to the loading concentration.

[Optional] One of the following temperature-controlled water baths or equivalent that can hold at 25°C:

•

Thermo Scientific Precision 35L Circulating Water Bath (holds five cartridges simultaneously)

•

SHEL LAB 22L Digital Circulating Water Bath (holds three cartridges simultaneously)

•

Thermo Fisher Scientific, catalog # TSCIR 35

•

Shel Lab, catalog # SWBC22

Thawing the cartridge.

Equipment for Manual Denature and Dilute (Optional)

For information on how to manually denature and dilute prepared libraries for sequencing on NextSeq 1000/2000, refer to the Denature and Dilute Protocol Generator.