Prepare and Add Custom Primers

Custom primers are diluted using HT1, and then added to the custom wells in the NextSeq 1000/2000 reagent cartridge. Make sure that the reagent cartridge is thawed and inspected before proceeding.

If using custom or third-party libraries alongside PhiX or Illumina libraries, prepare your custom read primers by spiking into HP21 and custom index primers by spiking into BP14.

HT1, HP21, and BP14 are included in the NextSeq 1000/2000 primer kits. Each kit configuration includes different reagents. HT1 is not included in the NextSeq 1000/2000 sequencing reagent kit and is offered as a standalone reagent for purchase. To verify the appropriate kit option for your libraries, refer to Kit Configurations.