Standard SBS Sequencing Protocol

This section provides step-by-step instructions on how to prepare standard SBS consumables, dilute libraries, and set up a sequencing run in one of four run modes. Cloud, Hybrid, and Local mode use DRAGEN or BaseSpace Sequence Hub apps, while Standalone mode is a standalone run intended for generating CBCL data only for custom analysis workflows.

When handling reagents and other chemicals, wear safety glasses, a lab coat, and powder-free gloves.

Make sure that you have the required consumables and equipment before starting a protocol. Refer to Consumables & Equipment.

Follow the protocols in the order shown, using the specified volumes, temperatures, and durations.