Sequencing Consumables

Sequencing on the NextSeq 1000/2000 system requires one of the following consumable kits:

Kit Configurations

Kit Name

Available Configurations

NextSeq 1000/2000 P1 XLEAP-SBS Reagent Kit

• 100-cycle
• 300-cycle
• 600-cycle

NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit

• 100-cycle
• 200-cycle
• 300-cycle
• 600-cycle

NextSeq 2000 P3 XLEAP-SBS Reagent Kit*
• 100-cycle
• 200-cycle
• 300-cycle

NextSeq 2000 P4 XLEAP-SBS Reagent Kit*
• 50-cycle
• 100-cycle
• 200-cycle
• 300-cycle

NextSeq 1000/2000 P1 Standard SBS Reagent Kit

• 100-cycle
• 300-cycle
• 600-cycle

NextSeq 1000/2000 P2 Standard SBS Reagent Kit

• 100-cycle
• 200-cycle
• 300-cycle
• 600-cycle

NextSeq 2000 P3 Standard SBS Reagent Kit*
• 50-cycle
• 100-cycle
• 200-cycle
• 300-cycle

*Only compatible with the NextSeq 2000 Sequencing System.

The reagent kit includes the cartridge and flow cell for sequencing, and RSB with Tween 20. When you receive your reagent kit:

• Promptly store components at the indicated temperatures to ensure proper performance.
• Do not open any silver foil bag until instructed to do so.
• Store cartridges in their box to avoid tearing or puncturing the foil bag.
• Store cartridges with the arrows pointing up.

If cartridge label is not facing up, sequencing data is negatively impacted.

Kit Components

Consumable

Quantity

Storage Temperature

Dimensions

Cartridge

1

-25°C to -15°C
• Standard SBS 100-cycle, 200-cycle, or 300-cycle cartridges:

29.2 cm × 17.8 cm × 12.7 cm (11.5 in × 7 in × 5 in)

• Standard SBS 600-cycle, and all XLEAP-SBS cartridges:

33.2 cm x 17.8 cm x 12.7 cm (13.1 in × 7 in × 5 in)

Flow cell

1

2°C to 8°C*

21.6 cm × 12.7 cm × 1.9 cm
(8.5 in × 5 in × 0.75 in)

RSB with Tween 20*

1

2°C to 8°C

4 cm x 6.6 cm x 5 cm

(1.6 in x 2.6 in x 2 in)

*Shipped at room temperature.

Both consumables have identifiers for tracking and ensuring compatibility. The cartridge and flow cell use RFIDradio-frequency identification.

The flow cell is a patterned, single-lane flow cell. A plastic cartridge encases the glass-based flow cell. A gray tab covers and protrudes from the flow cell to ensure safe handling.

  1. Plastic cartridge
  2. Flow cell
  3. Gray tab

Millions of nanowells cover the inner surface of the flow cell. Clusters are generated in the nanowells, from which the sequencing reaction is then performed. The patterned arrangement of the nanowells increases output reads and data.

The sequencing reagent cartridge is prefilled with clustering, sequencing, paired-end, and indexing reagents. A foil-sealed reservoir is reserved for libraries, and a slot on the front is reserved for the flow cell.

  1. Library reservoir
  2. Flow cell slot
  3. Arms
  4. Drainage plug

The cartridge contains all consumables for a run: reagents, library, and flow cell. The library and flow cell are loaded into the thawed cartridge, which is then loaded onto the instrument. After the run begins, reagents and library are automatically transferred from the cartridge to the flow cell.

The cartridge contains pumps, valves, and all fluidics for the system, including an underside reservoir to collect used reagents. The cartridge is discarded after a run, so instrument washes are not necessary.

The label on the cartridge indicates how many cycles are analyzed, not how many cycles are performed. The flow cell is compatible with any number of cycles and any read type.

All XLEAP-SBS cartridges include an extra 38 cycles. All standard SBS cartridges include an extra 38 cycles except for the 300-cycle cartridge in the NextSeq 2000 P3 Standard SBS Reagent Kit, which includes an extra 27 cycles. For example, the 200-cycle cartridge in the NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit provides sufficient reagents for up to 238 cycles of sequencing. For information on how many cycles to sequence, refer to Number of Cycles in a Read.

The following table describes the symbols on the consumable or consumable packaging.

Symbol

Description

The date the consumable expires. For best results, use the consumable before this date.

Indicates the manufacturer (Illumina).

The intended use is Research Use Only (RUO).

Indicates the batch code to identify the batch or lot that the consumable was manufactured in.¹

Indicates a health hazard.

Storage temperature range in degrees Celsius. Store the consumable within the indicated range.²