Network Considerations

The NextSeq 1000/2000 Sequencing System is designed for use with a network. The NextSeq 1000/2000 control computer runs Linux with SELinux enabled. The NextSeq 1000/2000 does not support encryption being enabled.

Performing a run in manual mode requires a network connection to transfer run data to a network storage location. Illumina recommends saving run data to an external or network drive. The local hard drive on the NextSeq 1000/2000 Sequencing System is intended for temporary storage before data is transferred automatically.

Security and Networking

It is recommended that you review the Product Security Portal to keep your instrument secure. These best practices include, for example, enabling firewalls and using appropriate account settings.

Network Connections

Use the following recommendations to install and configure a network connection:

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Use a dedicated 1 gigabit connection between the instrument and your local data management system. This connection can be made directly or through a managed network switch.

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Required bandwidth for a connection is as follows.

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200 Mb/s/instrument intranet bandwidth for local storage.

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5 Mb/s/instrument minimum internet bandwidth to support download of NextSeq 1000/2000 Control Software and DRAGEN Workflows (~15 GB). Download times out after 6 hours. 35 Mb/s/instrument internet bandwidth is required to support download within 1 hour.

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10 Mb/s/instrument internet bandwidth for BaseSpace Sequence Hub cloud storage (including Illumina Proactive Support).

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5 Mb/s/system internet bandwidth for run monitoring or Illumina Proactive Support only.

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Switches must be managed.

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Network equipment such as switches must have a minimum capacity of 1 gigabit per second.

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Calculate the total capacity of the workload on each network switch. The number of connected instruments and ancillary equipment, such as a printer, can impact capacity.

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If possible, isolate sequencing traffic from other network traffic.

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Illumina recommends the use of CAT-6 cables (minimum requirement is CAT-5e). Make sure that required cables are available before starting installation.

Network Support

Illumina does not install or provide technical support for network connections.

Review network architecture for potential compatibility risks with the Illumina system, including the following factors:

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Potential IP address conflicts—The NextSeq 1000/2000 Sequencing System assigns random internal IP addresses within range of 169.254.0.0/16, which can cause system failure if there are conflicts. NextSeq 1000/2000 Control Software v1.2.0 and later includes a virtual network interface named Docker with the default IP address of 172.17.0.1/16.

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IP assignment—The NextSeq 1000/2000 Sequencing System supports DHCP or Static IP assignment.

Control Computer Connections

The following tables describe the network ports and domains of the control computer. Use these tables as a reference when setting up your network.

Internal Connections

Connection	Value	Purpose
OS Designation	enp5s0	Communication between internal components (Do not configure or change after installation.)
Domain	localhost:*	All ports for localhost-to-localhost communication, which are needed for interprocess communication.
Port	8081	Real-Time Analysis
	8080	NextSeq 1000/2000 Control Software
	29644	Universal Copy Service (UCS)

Outbound Connections

Connection	Value	Purpose
OS Designation	enp6s0	Preferred Ethernet port for internet (right port when viewed from back of the instrument)
	enp2s0	Preferred Ethernet port for optional second network connection such as NAS or other network storage (left port when viewed from back of the instrument)
Port	443	BaseSpace Sequence Hub or Illumina Proactive configuration

Regional Platform Domains

For the regional platform domains that provide access from Universal Copy Service to (BaseSpace Sequence Hub) and Illumina Proactive, refer to Illumina Control Computer Security and Networking.