Clusters Passing Filter

During the run, RTA filters raw data to remove reads that do not meet the data quality threshold. Overlapping and low-quality clusters are removed.

For two-channel analysis, RTA uses a population-based system to determine the chastity of a base call. Clusters pass filter (PF) when no more than one base call in the first 25 cycles has a chastity of < 0.63. Clusters that do not pass filter are not base called.