Contents and Storage

The sequencing consumables required to run the NextSeq are provided separately in a single-use kit. Each kit includes one flow cell, a reagent cartridge, a buffer cartridge, and library dilution buffer. When you receive NextSeq 500/550 Kit:

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Do not open the foil package of the flow cell until instructed to do so.

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Promptly store components at the indicated temperatures to ensure proper performance.

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Store cartridges so that the package labels face up.

Consumable	Quantity	Storage Temperature	Description
reagent cartridge	1	-25°C to -15°C	Contains clustering and sequencing reagents
buffer cartridge	1	15°C to 30°C	Contains buffer and wash solution
HT1	1	-25°C to -15°C	Hybridization buffer
flow cell	1	2°C to 8°C*	Single-use flow cell

*Shipped at room temperature for NextSeq v2.5 Reagents kits

Reagents are sensitive to light. Store the reagent cartridge and buffer cartridge in a dark location away from light.

The flow cell, reagent cartridge, and buffer cartridge use radio-frequency identification (RFID) for accurate consumable tracking and compatibility.

All other kits include dual-index sequencing primers and NaOCI in the prefilled cartridge. No additional steps are required.