Denature and Dilute Libraries

Denature and dilute your libraries to the following loading volume and concentration.

Kit Type

Loading Volume

Loading Concentration

High Output

1.3 ml

1.8 pM

Mid Output

1.3 ml

1.5 pM

In practice, loading concentration can vary depending on library preparation and quantification methods. For instructions, see the NextSeq System Denature and Dilute Libraries Guide (document # 15048776).