Flow Cell Overview
Flow Cell Cartridge
- Lane pair A—Lanes one and three
- Lane pair B—Lanes two and four
- Flow cell cartridge frame
The flow cell is a glass-based substrate on which clusters are generated and the sequencing reaction is performed. The flow cell is encased in a flow cell cartridge.
The flow cell contains four lanes that are imaged in pairs.
| • | Lanes one and three (lane pair A) are imaged at the same time. |
| • | Lanes two and four (lane pair B) are imaged when imaging of lane pair A is complete. |
Although the flow cell has four lanes, only a single library or set of pooled libraries is sequenced on the flow cell. Libraries are loaded onto the reagent cartridge from a single reservoir and transferred automatically to the flow cell to all four lanes.
Each lane is imaged in small imaging areas called tiles.
