Checks for Sequencing Runs
If a pre-run check fails, the reagent cartridge RFID is not locked and can be used for a subsequent run. However, the flow cell, reagent cartridge, and buffer cartridge RFIDs will be locked during a control software initialization, which may be required to resolve an error. User must remove the flow cell, reagent cartridge, and buffer cartridge from the instrument prior to a system restart. Additionally, the consumable RFIDs are locked after the foil seals have been pierced. Once a flow cell RFID is read by the software, a 7 hour timer starts before the flow cell is considered locked and unusable.
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System Checks |
Recommended Action |
|---|---|
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Doors Closed |
Make sure that the compartment doors are closed. |
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Consumables Loaded |
Consumable sensors do not register. Make sure that each consumable is properly loaded. On the run setup screens, select Back to return to the loading step, and repeat run setup. |
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Required Software |
Critical components of the software are missing. Contact Illumina Technical Support. |
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Instrument Disk Space |
The instrument hard drive does not have sufficient disk space to perform a run. It is possible that data from a previous run did not transfer. Clear run data from the instrument hard drive. |
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Network Connection |
The network connection has been interrupted. Check network status and the physical network connection. |
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Network Disk Space |
The network server is full. |
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Temperature |
Recommended Action |
|---|---|
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Temperature |
Contact Illumina Technical Support. |
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Temperature Sensors |
Contact Illumina Technical Support. |
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Fans |
Contact Illumina Technical Support. |
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Imaging System |
Recommended Action |
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|---|---|---|---|---|---|---|---|
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Imaging Limits |
Contact Illumina Technical Support. |
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Z Steps-and-Settle |
Contact Illumina Technical Support. |
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Bit Error Rate |
Contact Illumina Technical Support. |
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Flow Cell Registration |
It is possible that the flow cell is not properly seated.
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Reagent Delivery |
Recommended Action |
|---|---|
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Valve Response |
Contact Illumina Technical Support. |
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Pump |
Contact Illumina Technical Support. |
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Buffer Mechanism |
Contact Illumina Technical Support. |
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Spent Reagents Empty |
Empty the spent reagents container and reload the empty container. |
