Prepare the Reagent Cartridge
| 1. | Remove the reagent cartridge from -25°C to -15°C storage. |
| 2. | Thaw in a room temperature water bath until thawed (~60 minutes). Do not submerge the cartridge. |
| 3. | Gently tap on the bench to dislodge water from the base, and then dry the base. |
[Alternate method] Thaw reagents overnight at 2°C to 8°C. Reagents require a minimum of 18 hours to thaw. At this temperature, reagents are stable up to one week.
| 4. | Invert the cartridge five times to mix reagents. |
| 5. | Inspect positions 29, 30, 31, and 32 to make sure that reagents are thawed. |
| 6. | Gently tap on the bench to reduce air bubbles. |
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation, ingestion, skin contact, and eye contact. Ventilation should be appropriate for handling of hazardous materials in reagents. Wear protective equipment, including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in accordance with applicable regional, national, and local laws and regulations. For additional environmental, health, and safety information, refer to the SDS at support.illumina.com/sds.html.
