RTA v2 Output Files
Images for each channel are passed in memory as tiles. Tiles are small imaging areas on the flow cell defined as the field of view by the camera. From these images, the software produces output as a set of quality-scored base call files and filter files. All other files are supporting output files.
File Type |
Description |
---|---|
Base call files (*.bcl) |
Each tile that is analyzed is included in an aggregated base call file for each lane and for each cycle. The aggregated base call file contains the base call and associated quality score for every cluster in that lane. |
Filter files (*.filter) |
Each tile produces filter information that is aggregated into one filter file for each lane. The filter file specifies whether a cluster passes filters. |
Cluster location files (*.locs) |
Cluster location files contain the X,Y coordinates for every cluster in a tile. A cluster location file is generated for each lane during template generation. |
Base call index files (*.bci) |
A base call index file is produced for each lane to preserve the original tile information. The index file contains a pair of values for each tile, which are tile number and number of clusters for that tile. |
RTA v2 provides real-time metrics of run quality stored as InterOp files. InterOp files are a binary output containing tile, cycle, and read-level metrics