RTA v2 Output Files

Images for each channel are passed in memory as tiles. Tiles are small imaging areas on the flow cell defined as the field of view by the camera. From these images, the software produces output as a set of quality-scored base call files and filter files. All other files are supporting output files.

File Type

Description

Base call files (*.bcl)

Each tile that is analyzed is included in an aggregated base call file for each lane and for each cycle. The aggregated base call file contains the base call and associated quality score for every cluster in that lane.

Filter files (*.filter)

Each tile produces filter information that is aggregated into one filter file for each lane. The filter file specifies whether a cluster passes filters.

Cluster location files (*.locs)

Cluster location files contain the X,Y coordinates for every cluster in a tile. A cluster location file is generated for each lane during template generation.

Base call index files (*.bci)

A base call index file is produced for each lane to preserve the original tile information. The index file contains a pair of values for each tile, which are tile number and number of clusters for that tile.

Output files are used for downstream analysis in BaseSpace. Alternatively, use bcl2fastq conversion software for FASTQ conversion and third-party analysis solutions. NextSeq files require bcl2fastq v2.0, or later. For the latest version of bcl2fastq, visit the NextSeq downloads page on the Illumina website.

RTA v2 provides real-time metrics of run quality stored as InterOp files. InterOp files are a binary output containing tile, cycle, and read-level metrics, and are required to view real-time metrics using the Sequencing Analysis Viewer (SAV). For the latest version of SAV, visit the SAV downloads page on the Illumina website.