Set Up a Run on the Instrument

1. Prepare a new reagent cartridge.
2. If the saved flow cell was stored, allow it to reach room temperature (15–30 minutes).
3. Clean and load the saved flow cell.
4. Remove the spent reagents container and discard the contents appropriately, and then reload the empty container.
5. On the Run Setup screen, select from one of the following run modes:
Local Run Manager
Manual
6. [Optional] Select Use BaseSpace Sequence Hub Setting and select from one of the following options:
Run Monitoring and Storage
Run Monitoring Only

Enter your BaseSpace Sequence Hub user name and password.

7. Load the new buffer cartridge and reagent cartridge.
8. Select Next to proceed to the pre-run check and start the run.