Enter Run and Analysis Parameters in NCS (Manual Run Mode)
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1.
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Enter a run name of your preference. |
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2.
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[Optional] Enter a library ID of your preference. |
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3.
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From the Recipe drop-down list, select a recipe. Only compatible recipes are listed. |
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4.
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Select a read type, either Single-Read or Paired-End. |
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5.
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Enter the number of cycles for each read in the sequencing run. |
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Read 1—Enter a value up to 151 cycles. |
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Read 2—Enter a value up to 151 cycles. This value is typically the same number of cycles as Read 1. |
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Index 1—Enter the number of cycles required for the Index 1 (i7) primer. |
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Index 2—Enter the number of cycles required for the Index 2 (i5) primer. |
The control software confirms your entries using the following criteria:
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Total cycles do not exceed the maximum cycles allowed |
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Cycles for Read 1 are greater than the 5 cycles used for template generation |
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Index Read cycles do not exceed Read 1 and Read 2 cycles |
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6.
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[Optional] If you are using custom primers, select the checkbox for the primers used. For more information, see NextSeq Custom Primers Guide (document # 15057456). |
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Read 1—Custom primer for Read 1. |
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Read 2—Custom primer for Read 2. |
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Index 1—Custom primer for Index 1. |
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Index 2—Custom primer for Index 2. |
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7.
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Set the output folder location for the current run. Select Browse to navigate to a network location. |
For information on output folder requirements, see Set Output Folder Location.
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8.
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Select Browse to navigate to a sample sheet. |
Systems configured for manual mode with Run Monitoring and Storage in BaseSpace Sequencing Hub require a sample sheet.
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9.
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Select Purge consumables for this run. |
The setting purges consumables automatically after the current run.
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11.
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[Optional] Select the Edit icon to change run parameters. |
