Scanning (NextSeq 550 only)
The information in this section only applies to the NextSeq 550.
To perform a scan on the NextSeq 550, you need the following run components:
| • | A hybridized and stained BeadChip |
| • | The reusable BeadChip adapter |
| • | Decode map (DMAP) files for the BeadChip you are using |
| • | A manifest file for the BeadChip you are using |
| • | A cluster file for the BeadChip you are using |
Output files are generated during the scan and then queued for transfer to the specified output folder.
Perform analysis using the BlueFuse Multi software, which requires that scanning data are available in a genotype call (GTC) file format. By default, the NextSeq 550 generates normalized data and associated genotype calls in the format of a GTC file. Optionally, you can configure the instrument to generate additional intensity data (IDAT) files. For more information, see BeadChip Scan Configuration.
