Starting the Instrument
1. | Turn on the power toggle switch to the I (on) position. |
Power Switch Located on Back of Instrument
To start the NextSeq 550Dx instrument in research mode, see Starting the NextSeq 550Dx Instrument.
2. | Press the power button above the reagent compartment. The power button turns on the instrument power and starts the integrated instrument computer and software. |
Power Button Located on Front of Instrument
3. | Wait until the operating system has finished loading. |
The NextSeq Control Software (NCS) launches and initializes the system automatically. After the initialization step is complete, the Home screen opens.
4. | If your system has been configured to require login credentials, wait for the system to load, and then log on to the operating system. If necessary, consult your facility administrator for the user name and password. |

Turn on the power toggle switch to the I (on) position.
Power Switch Located on Back of Instrument
1. | Press the power button above the reagent compartment. The power button turns on the instrument power and starts the integrated instrument computer and software. |
By default, the instrument boots into diagnostic mode.
Power Button Located on Front of Instrument
2. | Wait until the operating system has finished loading. |
The NextSeq 550Dx Operating Software (NOS) launches and initializes the system automatically. After the initialization step is complete, the Home screen opens.
3. | Enter your Local Run Manager user name and password. |
For information on Local Run Manager passwords, see the NextSeq 550Dx Instrument Reference Guide (document # 1000000009513).
4. | Select Login. The Home screen opens, with Sequence, Local Run Manager, Manage Instrument, and Perform Wash icons. |
5. | Use the Reboot to RUO command in NOS to safely shut down the instrument and reboot to research mode. |
• | Select Manage Instrument. |
• | Select Reboot / Shutdown. |
• | Select Reboot to RUO. |
6. | Wait until the operating system has finished loading. |
The NCS launches and initializes the system automatically. After the initialization step is complete, the Home screen opens.
7. | If your system has been configured to require login credentials, log in to Windows using the user name and password for your site. |
If you are not sure what mode the instrument is in, see Instrument Mode Indicators.