Prepare for a Manual Post-Run Wash

User-Supplied Consumables

Volume and Description
• NaOCl

1 ml, diluted to 0.12%

Loaded onto the reagent wash cartridge (position #28)
• 100% Tween 20
• Laboratory-grade water

Used to make 125 ml 0.05% Tween 20 wash solution

Loaded onto the buffer wash cartridge (center reservoir)

Always use a fresh dilution of NaOCl prepared within the last 24 hours. If you make a volume larger than 1 ml, store the remaining dilution at 2°C to 8°C for use within the next 24 hours. Otherwise, discard the remaining dilution of NaOCl.

1. Combine the following volumes in a microcentrifuge tube to result in 1 ml 0.12% NaOCl:
• 5% NaOCl (24 µl)
• Laboratory-grade water (976 µl)
2. Invert the tube to mix.
3. Add 1 ml 0.12% NaOCl to the reagent wash cartridge. The correct reservoir is equivalent to position #28 on the prefilled cartridge.

Load NaOCl

4. Combine the following volumes to result in a 0.05% Tween 20 wash solution:

Original style buffer wash cartridge

• 100% Tween 20 (62 µl)
• Laboratory-grade water (125 ml)
• Add 125 ml wash solution to the center reservoir of the buffer wash cartridge.

New style buffer wash cartridge

• 100% Tween 20 (75 µl)
• Laboratory-grade water (150 ml)
• Add 150 ml wash solution to the center reservoir of the buffer wash cartridge.
5. Select Perform Wash, and then select Manual Post-Run Wash.