Load a Used Flow Cell and the Wash Cartridges

If a used flow cell is not present, load a used flow cell. Select Load, and then select Next.

Remove the spent reagents container and discard the contents in accordance with applicable standards.

This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation, ingestion, skin contact, and eye contact. Ventilation should be appropriate for handling of hazardous materials in reagents. Wear protective equipment, including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in accordance with applicable regional, national, and local laws and regulations. For additional environmental, health, and safety information, refer to the SDS at support.illumina.com/sds.html.

Slide the empty spent reagents container into the buffer compartment until it stops.

Remove the used buffer cartridge from the previous run, if present.

Load the buffer wash cartridge containing wash solution.

Remove the used reagent cartridge from the previous run, if present.

Load the reagent wash cartridge.

Select Next. The prewash check begins automatically.