Phasing Correction
During the sequencing reaction, each DNA strand in a cluster extends by one base per cycle. Phasing and prephasing occurs when a strand becomes out of phase with the current incorporation cycle.
| • | Phasing occurs when a base falls behind. |
| • | Prephasing occurs when a base jumps ahead. |
Phasing and Prephasing
- Read with a base that is phasing
- Read with a base that is prephasing
RTA corrects the effects of phasing and prephasing, which maximizes the data quality at every cycle throughout the run.
