Instrument Software

The NovaSeq 6000Dx with DRAGEN Server includes integrated applications that perform sequencing runs, analysis on-instrument and on the server, and other related functions. For more information on configuring instrument software, refer to System Configuration.

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NovaSeq Operating Software (NVOS)—Guides you through loading procedures, controls instrument operations, and displays statistics as the run progresses. NVOS operates the flow cell stage, dispenses reagents, controls fluidics, sets temperatures, captures images of clusters on the flow cell, and provides a visual summary of quality statistics.

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Real-Time Analysis (RTA)—Performs image analysis and base calling during a run. NovaSeq 6000Dx uses RTA3, which incorporates architecture, security, and other feature enhancements to optimize performance.

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Universal Copy Service (UCS)—Copies output files from RTA3 and NVOS to the output folder and to the DRAGEN Server throughout a run. If the Universal Copy Service is interrupted during a run, the service makes multiple attempts to reconnect and automatically resume data transfer.

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Illumina Run Manager—Plan runs, view planned runs, and review run results on the NovaSeq 6000Dx or remotely using a web browser. Illumina Run Manager also controls user and application permissions.

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Illumina DRAGEN Server for NovaSeq 6000Dx— When sequencing on the NovaSeq 6000Dx is complete, the analysis begins on the DRAGEN Server. Analysis on the DRAGEN Server and sequencing on the instrument can run concurrently.