User-Supplied Consumables & Equipment
The following sections provide information on the required user-supplied consumables and equipment. Additional user-supplied consumables are required for diluting and denaturing libraries. For more information, refer to Denature and Dilute Protocol Generator.
Consumables
|
Consumable |
Supplier |
Purpose |
|---|---|---|
|
Air filter |
Illumina, catalog # 20073109 |
Replacing air filter. NovaSeq X Series ships with one air filter and four replacements. |
|
Centrifuge bottle, 500 ml |
General lab supplier |
Diluting Tween 20 for a maintenance wash. |
|
Centrifuge tube, 50 ml |
General lab supplier |
Diluting NaOCl for a maintenance wash. |
|
Contec Polynit Heatseal wipes |
VWR, catalog #68310-176, or equivalent general lab supplier |
Cleaning and drying the flow cell and the flow cell stage. |
|
Disposable gloves, powder-free |
General lab supplier |
General purpose. |
|
Reagent grade NaOCl, 5% |
Sigma-Aldrich, catalog # 239305 |
Performing a maintenance wash. |
|
Pipette tips, 20 μl |
General lab supplier |
Pipetting for loading libraries. |
|
Pipette tips, 200 μl |
General lab supplier |
Pipetting for loading libraries. |
|
Pipette tips, 1000 μl |
General lab supplier |
Pipetting for loading libraries. |
|
Reagent or spectrophotometric-grade isopropyl alcohol (70%), 100 ml bottle |
General lab supplier |
Cleaning the flow cell stage. |
|
Tween 20 |
Sigma-Aldrich, catalog # P7949 |
Performing a maintenance wash. |
|
Water, laboratory-grade |
General lab supplier |
Diluting Tween 20 and sodium hypochlorite for a maintenance wash. |
|
Water conditioner |
Electron Microscopy Sciences, catalog # 60502-01, or equivalent general lab supplier |
Stabilizing water in water baths and preventing microbial growth. |
|
[Optional] EZwaste HD 20 L, HDPE, 83 mm Cap, 4x 1/16", 3x 1/4" OD Tube Fittings, 1x 1/4 or 3/8" ID Hose Barb and Filter |
VWR, catalog #76018-558, or equivalent general lab supplier |
Collecting bulk used reagent buffer waste. |
|
[Optional] NovaSeq X Series Custom Primer Buffer |
Illumina, catalog # 20100055 |
Performing the custom primer workflow. |
|
[Optional] PhiX Control v3 |
Illumina, catalog # FC-110-3001 |
Spiking in PhiX control. |
Equipment
|
Item |
Source |
|---|---|
|
Centrifuge |
General lab supplier |
|
General lab supplier |
|
|
Graduated cylinder, 500 ml, sterile |
General lab supplier |
|
Ice bucket |
General lab supplier |
|
Pipette, 20 µl |
General lab supplier |
|
Pipette, 200 µl |
General lab supplier |
|
Pipette, 1000 µl |
General lab supplier |
|
Refrigerator, 2°C to 8°C |
General lab supplier |
|
Tub, water baths* |
General lab supplier |
* Use a tub that can accommodate the reagent cartridges and the appropriate water level.
